Capacity:for1×109totalcellsOverview
CD326(EpCAM)MicroBeadscanbeusedforthe:
- positiveselectionordepletionofepithelialcellsorepithelialtumorcellsfromsingle-cellpreparationsoftissuesandorgans,
- enrichmentofcirculatingordisseminatedepithelialtumorcellsexpressingCD326fromperipheralblood,bonemarrow,orevenlymphnodes,SEROuseffusions,orstoolsamples1ofpatientswithcarcinomas,
- positiveselectionoffunctionalpluripotenthepaticProgenitorcells(hepaticstemcellsandhepatoblasts)fromfetal,neonatal,pediatric,andadultlivertissue2,3,
- positiveselectionoftumorexosomesfromperipheralblood4,
- enrichmentofexfoliatedepithelialcellsfromgastricaspiratesandstoolsamplesofpreterminfants5,
- purificationofprimarytumorcellcultures6.
Details
Backgroundinformation
CD326,alsoknownashumanepithelialantigen(HEA),epithelialcelladhesionmolecule(EpCAM),orepithelial-specificantigen(ESA)expressionisseeninthemajorityofcarcinomas.Itisexpressedoncellsofepithelialorigin,epithelium-derivedtumorcells7,circulatingtumorcells,andcancerstemcells.
Downstreamapplications
TumorcellsenrichedbyCD326(EpCAM)MicroBeadscanbecultured
8andanalyzedbyimmunocytochemistry
9,flowcytometry,ormolecular
BIOLOGytechniques,forexample,RT-PCR
10.
Columns
MS,LS,XS,orauto
MACS®Columns.