CSandDColumnsaresuitableforoptimaldepletionoflargecellnumbers.Byusingthedifferentflowresistorssuppliedwiththecolumns,theflowratecanbeadjusted,allowingefficientdepletionevenofcellsthatexpresstheseparation
Markerdimly.
CSColumnscanbeusedwiththeVario
MACS™,orSuperMACS™IISeparator.
DColumnsfitintotheSuperMACSIISeparator.
ThematrixofCSandDColumnsiscomposedofferromagneticfiberscoveredwithacell-friendlycoating.WhenplacedinthemagneticfieldofaMACS
®Separator,thefibersamplifythemagneticfield,thusinducingahighgr
ADIentwithinthecolumn.ThisiscrucialforisolationofcellswhichareonlyminimallylabeledwithMACS
®MicroBeads,leavingenoughepitopesfreeforconcurrentantibodystaining.Thespacebetweenthefibersisseveraltimeslargerthanprimaryandmostculturedcells.Thisallowsthecellstofreelyflowthroughthecolumn.Magneticallylabeledcellsareheldins
USPensionwithinthecolumnanddonotactually“bind”thecolumnmatrix.Thissuspensionminimizesstressonthecellsandallowsforefficientsterilewashingbyavoidingcellaggregation.
Thecolumnsareindividuallypackedinasterileway.