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Gibco/FreeStyle CHO Expression Medium/6 x 1,000 mL/12651022
  • Gibco/FreeStyle CHO Expression Medium/6 x 1,000 mL/12651022

Gibco/FreeStyle CHO Expression Medium/6 x 1,000 mL/12651022

价格: ¥6576.00 市场价: 10960.00

货号: 12651022
品牌: Gibco
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    • Description

      FreeStyle™CHOExpressionMediumisananimalorigin–free,chemicallydefined,protein-freemediumspecificallydevelopedtosupportthegrowthandtransienttransfectionofCHO-ScellsinsUSPensionwithoutadaptation.

      Thismediumrequiressupplementationwith8mMglutaminebeforeuse.Gibco®FreeStyle™CHOExpressionMediumfeatures:

      •Formulationwithouthypoxanthineandthymidineforusewithorwithoutdhfrsystems
      ABIlitytosupporthigh-densitysuspensiongrowthandtransienttransfectionofCHO-Scells
      •Animalorigin–free,protein-freeandchemicallydefinedformulation
      •EasyuseaspartoftheFreeStyle™MAXCHOExpressionSystemforproteinproduction
      •Scalabilityinspinnerflasksandbioreactors

      Formulationwithouthypoxanthineandthymidineforusewithorwithoutdhfrsystems
      Gibco®FreeStyle™CHOExpressionMediumismadewithouthypoxanthineandthymidineforuseindihydrofolatereductase(dhfr)amplificationsystems.Non-dhfrsystemsrequiretheadditionofHTSupplementbeforeuse.

      Abilitytosupporthigh-densitysuspensiongrowthandtransienttransfectionofCHO-Scells
      Gibco®FreeStyle™CHOcellsinsuspensioncanbecultureddirectlyintoGibco®FreeStyle™CHOExpressionMediumwithoutadaptation.Cellsadaptedtoothercommerciallyavailableserum-freemediacanbesubcultureddirectlyintoGibco®FreeStyle™CHOExpressionMedium,usuallywithoutanyfurtheradaptation.Cellsusuallyrequireadaptationfromserum-containingformulations.CellsculturedinGibco®FreeStyle™CHOExpressionMediumcanbetransfectedusinglipid-basedtransfectionreagents,suchasFreestyle™MAXreagent.

      Animalorigin–free,protein-freeandchemicallydefinedformulation
      Gibco®FreeStyle™CHOExpressionMediumisanimalorigin–free,protein-free,andchemicallydefined,allowingforeasierpurificationofyourproteinofinterest.Gibco®chemicallydefinedmediacontainnoproteins,hydrolysates,orcomponentsofunknowncomposition.

      EasyuseaspartoftheFreeStyle™MAXCHOExpressionSystemforproteinproduction
      TheFreeStyle™MAXCHOExpressionSystemisabreakthroughtechnologyforrapid(seefigure)andhigh-yieldmammalianproteinproductionin30mLand1Lbatchsizes(seefigure).TheFreeStyle™MAXCHOExpressionSystemcombinesGIBCO®FreeStyle™media,FreeStyle™MAXreagent,andeitherFreeStyle™CHO-ScellsorFreeStyle™293-Fcellsinonekit.

      Scalabilityinspinnerflasksandbioreactors
      ProteinproductionusingGibco®FreeStyle™CHOExpressionMediumcanbescaledupinspinnerflasksorbioreactors.Theappropriatespinnerorimpellerspeedandseedingdensityshouldbeoptimizedforeachsystem.Dependingontheimpellerdesignandspeed,itmaybenecessarytosupplementGibco®FreeStyle™CHOExpressionMediumwithadditionalPluronic®F-68toavoidsheerstressintheculture.

      cGMPmanufacturingandqualitysystem
      Gibco®FreeStyle™CHOExpressionMediumismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
      ForResearchUseOnly.Notforuseindiagnosticprocedures.

      Figures

      Specifications

      CellLine:CHO, FreeStyle™CHO-SCells
      Classification:ChemicallyDefined, Protein-Free, Serum-Free
      CultureType:SuspensionCellCulture
      EndotoxinLevel:≤0.25EU/ml
      Form:Liquid
      Format:Bottle(s)
      Glutamine:NoGlutamine
      PhenolRedIndicator:NoPhenolRed
      ProductLine:Freestyle™, Gibco®
      ProductSize:1,000mL
      SerumLevel:Serum-Free
      GreenFeatures:Sustainablepackaging
      ShippingCondition:RoomTemperature

      Contents&storage

      Storageconditions:2°Cto8°C.Protectfromlight
      Shippingconditions:Ambient
      Shelflife:12monthsfromdateofmanufacture
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