Description
AdvancedRPMI(RoswellParkMemorialInstitute)1640isawidelyusedbasalmediumthatallowsthecultureofmammaliancellswithreducedFetalBovineSerum(FBS)supplementation.ComparedtoclassicRPMI1640,serumsupplementationcanbereducedby50-90%withnochangeingrowthrateormorphology.
iCellssuccessfullyculturedinAdvancedRPMI1640,withnoadaptation,includeSp2,Vero,Raji,Daudi,andJurkat.
ThisAdvancedRPMI1640ismanufacturedasfollows:
With | Without |
•Glucose | •L-glutamine |
•NonEssentialAminoAcids | •HEPES |
•Sodiumpyruvate | |
Thecompleteformulationisavailable.
Gibco®AdvancedRPMI1640isuniquefromothermediaduetoadditionofthefollowingingredientstoallowforserumreduction:ethanolamine,glutathione,ascorbicacid,insulin,transferrin,AlbuMAX®Ilipid-richbovineserumalbuminforcellculture,andthetraceelementssodiumselenite,ammoniummetavanadate,cupricsulfate,andmanganouschloride.
ProductUseForResearchUseOnly.Notintendedforanimalorhumandiagnosticortherapeuticuse.
cGMPManufacturingandQualitySystemGibco®AdvancedRPMI1640ismanufacturedatacGMPcompliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standards.
AdvancedRPMI1640requiressupplementationwith1-5%FetalBovineSerumand4mML-glutamineorGlutaMAX™supplement.Manycelllinesdonotrequireadaptationtothismedia.TheFBSconcentrationmustbeoptimizedforeachcelllinetoobtainmaximumserumreduction.AdvancedRPMI1640usesasodiumbicarbonatebuffersystem(3.7g/L)andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
iAdvancedMedia:ReducedSeraSolutions.Quest,2004.1.3:26-30.
ForResearchUseOnly.Notforuseindiagnosticprocedures.