Description
TrypLE™Expressisananimalorigin-free,recombinantenzymeusedfordissociatingawiderangeofadherentmammaliancells,includingCHO,HEK293,A529,primaryhumankeratinocytes,andembryonicstemcells.TrypLE™ExpresscleavespeptidebondsontheC-terminalsidesoflysineandarginine,andisadirectreplacementfortrypsin.Itsexceptionalpurityincreasesspecificityandreducesdamagetocellsthatcanbecausedbyotherenzymespresentinsometrypsinextracts.WeofferavarietyofTrypLE™formulationsforarangeofcellcultureapplications.Comparedtotrypsinandotherdissociationreagents,TrypLE™Expressis:
•Gentleoncells
•Roomtemperature-stable
•Easytouse
•Animalorigin-free(AOF)
ThisTrypLE™Expressismodifiedasfollows: TheformulationofTrypLE™solutionsisavailable,althoughwekeeptheenzymeconcentrationconfidential.
GentleoncellsTrypLE™Express"exceptionalpurityincreasesspecificityduetotheactionofasingleenzyme.Thisreducesdamagecausedbycleavagefrommultipleenzymesintrypsinandotherextracts.
Roomtemperature-stableTrypLE™Expressremainsstablefor24monthsatroomtemperatureor2–8°C,makingstorageandhandlingeasyandconvenient.
EasytouseTrypLE™Expresscanbedirectlysubstitutedfortrypsininexistingprotocols.Inaddition,dilutionaloneinactivatesTrypLE™Express,avoidingtheneedfortrypsininhibitors,suchasFBS.
Animalorigin-free(AOF)Unlikeporcinetrypsin,TrypLE™Expressisfreeofanimal-derivedcomponents.
cGMPmanufacturingandqualitysystemTrypLE™ExpressismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
ForResearchUseOnly.Notforuseindiagnosticprocedures.