Description
MEMα(MinimumEssentialMediumα)iswidelyusedformammaliancellcultureaswellasselectionfortransfectedDHFR-negativecells.MEMαcanbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingkeratinocytes,primaryratastrocytes,andhumanmelanomacells.WeofferavarietyofMEMαmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisMEMαismodifiedasfollows:With |
•Ribonucleosides |
•Deoxyribonucleosides |
•PhenolRed |
Thecompleteformulationisavailable.
UsingMEMαMEMαisamodificationofMinimumEssentialMedium(MEM)thatcontainsnon-essentialaminoacids,sodiumpyruvate,lipoicacid,vitaminB
12,biotin,andascorbicacid.MEMαisavailablewithoutnucleosidesforuseasaselectionmediumforDG44andotherDHFR-negativecells.ThisproductismadewithEarle’ssalts.MEMαcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMαrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).MEMαusesasodiumbicarbonatebuffersystem(2.2g/L),andthereforerequiresa5–10%CO
2environmenttomaintainphysiologicalpH.
cGMPmanufacturingandqualitysystemMEMαismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferanidenticalMEMαproductmadeinourScotlandfacility(22571-020).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
ForInVitroDiagnosticUse.