Description
Dulbecco"sModifiedEagleMedium
:NutrientMixtureF-12(DMEM/F-12)isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinDMEM/F-12includeMDCK,glialcells,fibroblasts,humanendothelialcells,andratfibroblasts.Weofferavarietyof
Gibco®DMEM/F-12modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisDMEM/F-12ismodifiedasfollows:
With | Without |
•L-glutamine | •HEPES |
Thecompleteformulationisavailable.
Gibco®DMEM/F-12isa1
:1mixtureofDMEMandHam"sF-12.ThisformulationcombinesDMEM"shighconcentrationsofglucose,aminoacidsandvitaminswithF-12"swidevarietyofcomponents.
ProductIntendedUseFor
invitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.
cGMPManufacturingandQualitySystemGibco®DMEM/F-12ismanufacturedatacGMPcompliantfacility,locatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferacomparableGibco®DMEM/F-12productmadeinourScotlandfacility(32500-035).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
DMEM/F-12containsnoproteins,lipids,orgrowthfactors.Therefore,DMEM/F-12mayrequiresupplementation,commonlywith10%FetalBovineSerum(FBS).DMEM/F-12usesasodiumbicarbonatebuffersystemandthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
PowderformsofGibco®cellculturemediumrequiresodiumbicarbonatesupplementation,pHadjustment,andfiltrationatthetimeofpreparation(seeprotocolfordetails).
ForInVitroDiagnosticUse.