Description
AdvancedMEM(MinimumEssentialmedium)isawidelyusedbasalmediumthatallowsthecultureofmammaliancellswithreducedFetalBovineSerum(FBS)supplementation.ComparedtoclassicMEM,serumsupplementationcanbereducedby50-90%withnochangeingrowthrateormorphology.
iCellssuccessfullyculturedinAdvancedDMEM/F-12,withnoadaptation,includePK-15,MDCK,Vero,WI-38andHep-2.
ThisAdvancedMEMismanufacturedasfollows:
With | Without |
•Glucose | •L-glutamine |
•NonEssentialAminoAcids | •HEPES |
•Sodiumpyruvate | |
Thecompleteformulationisavailable.
Gibco®AdvancedMEMisuniquefromothermediaduetoadditionofthefollowingingredientstoallowforserumreduction:ethanolamine,glutathione,ascorbicacid,insulin,transferrin,AlbuMAX®Ilipid-richbovineserumalbuminforcellculture,andthetraceelementssodiumselenite,ammoniummetavanadate,cupricsulfate,andmanganouschloride.
ProductUseForResearchUseOnly.Notintendedforanimalorhumandiagnosticortherapeuticuse.
cGMPManufacturingandQualitySystemGibco®AdvancedMEMismanufacturedatacGMPcompliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standards.
AdvancedMEMrequiressupplementationwith1-5%FetalBovineSerumand4mML-glutamineorGlutaMAX™supplement.Manycelllinesdonotrequireadaptationtothismedia.TheFBSconcentrationmustbeoptimizedforeachcelllinetoobtainmaximumserumreduction.AdvancedDMEMusesasodiumbicarbonatebuffersystem(3.7g/L)andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
iPaulPrice,BradStiles,DavidStaines,EthelEvege,FemiFatunmbi,KateWagner,LoriNestler,DavidJayme.AdvancedMediaasAlternativestoClassicalBasalMedia.FOCUS,2003.25.2:3-6.
ForResearchUseOnly.Notforuseindiagnosticprocedures.