Description
AdvancedDMEM(Dulbecco"sModifiedEagleMedium)isawidelyusedbasalmediumthatallowsthecultureofmammaliancellswithreducedFetalBovineSerum(FBS)supplementation.ComparedtoclassicDMEM,serumsupplementationcanbereducedby50–90%withnochangeingrowthrateormorphology.CellssuccessfullyculturedinAdvancedDMEM,withnoadaptation,includeMDBK,HepG2,COS-7,A549,MDCK,WI-38,andVero.
ThisAdvancedDMEMismanufacturedasfollows:With | Without |
•Highglucose | •L-glutamine |
•NonEssentialAminoAcids | |
•Sodiumpyruvate | |
Thecompleteformulationisavailable.
UsingAdvancedDMEMAdvancedDMEMisuniquefromothermediaduetotheadditionofthefollowingingredientstoallowforserumreduction:ethanolamine,glutathione,ascorbicacid,insulin,transferrin,AlbuMAX®Ilipid-richbovineserumalbuminforcellculture,andthetraceelementssodiumselenite,ammoniummetavanadate,cupricsulfate,andmanganouschloride.AdvancedDMEMrequiressupplementationwith1–5%FetalBovineSerumand4mML-glutamineorGlutaMAX™supplement.Manycelllinesdonotrequireadaptationtothismedia.TheFBSconcentrationmustbeoptimizedforeachcelllinetoobtainmaximumserumreduction.AdvancedDMEMusesasodiumbicarbonatebuffersystem(3.7g/L),andthereforerequiresa5–10%CO
2environmenttomaintainphysiologicalpH.
cGMPmanufacturingandqualitysystemForsupplychaincontinuity,wemanufactureAdvancedDMEMattwoseparatefacilitieslocatedinGrandIsland,NYandScotland,UK.BothsitesarecompliantwithcGMPmanufacturingrequirements,arecertifiedtoISO13485,andareregisteredwiththeFDAasmedicaldevicemanufacturers.
ForResearchUseOnly.Notforuseindiagnosticprocedures.