Description
Dulbecco"sModifiedEagleMedium(DMEM)isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinDMEMincludeprimaryfibroblasts,neurons,glialcells,HUVECs,andsmoothmusclecells,aswellascelllinessuchasHeLa,293,Cos-7,andPC-12.Weofferavarietyof
Gibco®DMEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisDMEMismodifiedasfollows:
With |
•LowGlucose |
•L-glutamine |
•SodiumPyruvate |
•HEPES |
Thecompleteformulationisavailable.
DMEMisuniquefromothermediaasitcontains4timestheconcentrationofaminoacidsandvitaminsthantheoriginalEagle"sMinimalEssentialMedium.DMEMwasoriginallyformulatedwithlowglucose(1g/L)andsodiumpyruvate,butisoftenusedwithhigherglucoselevels,withorwithoutsodiumpyruvate.
ProductUseForhuman
exvivotissueandcellcultureprocessingapplications.CAUTION:Whenusedasamedicaldevice,Federallawrestrictsthisdevicetosalebyorontheorderofaphysician.CustomersusingGibco®DMEMinamanufacturingprocess,whohaveasubmissionwiththeFDA,mayrequestaletterofauthorizationfromustoreferenceourTypeIIDrugMasterFile(DMF).
cGMPManufacturingandQualitySystemGibco®DMEMismanufacturedatacGMPcompliantfacility,locatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferanidenticalGibco®DMEMproductmadeinourScotlandfacility(22320-022).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
DMEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,DMEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).DMEMusesasodiumbicarbonatebuffersystem(3.7g/L)andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
ForInVitroDiagnosticUse.