Description
MinimumEssentialMedium(MEM)αiswidelyusedformammaliancellcultureaswellasselectionfortransfectedDHFRnegativecells.MEMαcanbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingkeratinocytes,primaryratastrocytes,andhumanmelanomacells.Weofferavarietyof
Gibco®MEMαmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisMEMαismodifiedasfollows:
With | Without |
•PhenolRed | •Ribonucleosides |
•L-glutamine | •Deoxyribonucleosides |
Thecompleteformulationisavailable.
Gibco®MEMαisamodificationofMinimumEssentialMedium(MEM)thatcontainsnon-essentialaminoacids,sodiumpyruvate,lipoicacid,vitaminB
12,biotin,andascorbicacid.MEMαisavailablewithoutnucleosidesforuseasaselectionmediumforDG44andotherDHFRnegativecells.ThisproductismadewithEarle’ssalts.
ProductIntendedUseFor
invitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.
cGMPManufacturingandQualitySystemGibco®MEMαismanufacturedatacGMPcompliantfacility,locatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferacomparableGibco®MEMαproductmadeinourScotlandfacility(12000-014).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
MEMαcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMαrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).MEMαusesasodiumbicarbonatebuffersystem(2.2g/L)andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.PowderformsofGibco®cellculturemediumrequiresodiumbicarbonatesupplementation,pHadjustment,andfiltrationatthetimeofpreparation(seeprotocolfordetails).
ForInVitroDiagnosticUse.