Description
MinimumEssentialMedium(MEM)αiswidelyusedformammaliancellcultureaswellasselectionfortransfectedDHFRnegativecells.MEMαcanbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingkeratinocytes,primaryratastrocytes,andhumanmelanomacells.Weofferavarietyof
Gibco®MEMαmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisMEMαismodifiedasfollows:
With | Without |
•Ribonucleosides | •SodiumBicarbonate |
•Deoxyribonucleosides | |
•L-glutamine | |
Thecompleteformulationisavailable.
Gibco®MEMαisamodificationofMinimumEssentialMedium(MEM)thatcontainsnon-essentialaminoacids,sodiumpyruvate,lipoicacid,B
12,biotin,andascorbicacid.MEMαisavailablewithoutnucleosidesforuseasaselectionmediumforDG44andotherDHFRnegativecells.ThisproductismadewithEarle’ssalts.
ProductIntendedUseFor
invitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.
cGMPManufacturingandQualitySystemGibco®MEMαismanufacturedatacGMPcompliantfacility,locatedinPaisley,Scotland,UK.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.Forsupplychaincontinuity,weofferacomparableGibco®MEMαproductmadeinourGrandIslandfacility(11900-024).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standards.
MEMαcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMαrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).MEMαusesasodiumbicarbonatebuffersystem(2.2g/L)andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
PowderformsofGibco®cellculturemediumrequiresodiumbicarbonatesupplementation,pHadjustment,andfiltrationatthetimeofpreparation(seeprotocolfordetails).