Gibco/RPMI 1640 Medium/10 x 500 mL/11875119

Description

RPMI1640MediumwasoriginallydevelopedtoculturehumanleukemiccellsinsUSPensionandasamonolayer.RoswellParkMemorialInstitute(RPMI)1640Mediumhassincebeenfoundsuitableforavarietyofmammaliancells,includingHeLa,Jurkat,MCF-7,PC12,PBMC,astrocytes,andcarcinomas.WeofferavarietyofRPMI1640Mediummodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.


Thecompleteformulationisavailable.

UsingRPMI
RPMI1640Mediumisuniquefromothermediabecauseitcontainsthereducingagentglutathioneandhighconcentrationsofvitamins.RPMI1640Mediumcontainsbiotin,vitaminB₁₂,andPABA,whicharenotfoundinEagle"sMinimalEssentialMediumorDulbecco"sModifiedEagleMedium.Inaddition,thevitaminsinositolandcholinearepresentinveryhighconcentrations.RPMI1640Mediumcontainsnoproteins,lipids,orgrowthfactors.Therefore,RPMI1640Mediumrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).RPMI1640Mediumusesasodiumbicarbonatebuffersystem(2.0g/L),andthereforerequiresa5–10%CO₂environmenttomaintainphysiologicalpH.

Productuse
Forinvitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.CustomersusingGibco®RPMI1640inamanufacturingprocess,whohaveasubmissionwiththeFDA,mayrequestaletterofauthorizationfromustoreferenceourTypeIIDrugMasterFile(DMF).

cGMPmanufacturingandqualitysystem
RPMI1640MediumismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferanidenticalRPMI1640productmadeinourScotlandfacility(21875-059).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.