Description
Ham"sF-12NutrientMixture(F-12)wasdesignedforserum-free,single-cellplatingofChineseHamsterOvary(CHO)cells.F-12hassincebeenusedforserum-freegrowthofCHOculturesaswellasserum-supplementedgrowthofothermammaliancells,includingchondrocytesandratprostateepithelialcells.WeofferavarietyofF-12modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisHam"sF-12ismodifiedasfollows: Thecompleteformulationisavailable.
UsingF-12Comparedtootherbasalmedia,F-12containsawidervarietyofcomponents,includingzinc,putrescine,hypoxanthine,andthymidine.F-12containsnoproteinsorgrowthfactors.Therefore,F-12requiressupplementation,commonlywith10%FetalBovineSerum(FBS).F-12usesasodiumbicarbonatebuffersystem(1.176g/L),andthereforerequiresa5–10%CO
2environmenttomaintainphysiologicalpH.
cGMPmanufacturingandqualitysystemForsupplychaincontinuity,wemanufactureF-12attwoseparatefacilities;onelocatedinGrandIsland,NYandtheotherinScotland,UK.BothsitesarecompliantwithcGMP-manufacturingrequirements,arecertifiedtoISO13485,andareregisteredwiththeFDAasmedicaldevicemanufacturers.