Description
MinimumEssentialMedium(MEM)isoneofthemostcommonlyusedofallcellculturemedia.MEMcanbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingHeLa,BHK-21,293,HEP-2,HT-1080,MCF-7,fibroblasts,andprimaryratastrocytes.Weofferavarietyof
Gibco®MEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisMEMismodifiedasfollows:
With | Without |
•Earle"ssalts | •L-glutamine |
•PhenolRed | •HEPES |
Thecompleteformulationisavailable.
MEM,developedbyHarryEagle,wasbasedonhisearlierformulationofBasalMediumEagle(BME).ManyothermodificationsofMEMfollowed,includingGlasgow’sMEM,MEMα,DMEM,andTemin’sModification.ThisMEMformulationismadewithoutcalciumforusewithsuspensioncultures.ThisMEMformulationcontainsEarle’ssaltsforuseinaCO
2incubator.ThisproductismadewithEarle’ssalts.
ProductIntendedUseFor
invitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthanthelabeledintendedusemaybeaviolationoflocallaw.
DualsitecGMPManufacturingandQualitySystemGibco®MEMismanufacturedatacGMPcompliantfacility,locatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferanidenticalGibco®MEMproductmadeinourScotlandfacility(11380-052).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
MEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).MEMusesasodiumbicarbonatebuffersystem(2.2g/L)andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.