Description
DMEM/F-12(Dulbecco"sModifiedEagleMedium/NutrientMixtureF-12)isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinDMEM/F-12includeMDCK,glialcells,fibroblasts,humanendothelialcells,andratfibroblasts.WeofferavarietyofDMEM/F-12modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisDMEM/F-12ismodifiedasfollows: Thecompleteformulationisavailable.
UsingDMEM/F-12DMEM/F-12isa1:1mixtureofDMEMandHam"sF-12.ThisformulationcombinesDMEM"shighconcentrationsofglucose,aminoacids,andvitaminswithF-12"swidevarietyofcomponents.DMEM/F-12containsnoproteins,lipids,orgrowthfactors.Therefore,DMEM/F-12mayrequiresupplementation,commonlywith10%FetalBovineSerum(FBS).DMEM/F-12usesasodiumbicarbonatebuffersystemandthereforerequiresa5–10%CO
2environmenttomaintainphysiologicalpH.
cGMPmanufacturingandqualitysystemDMEM/F-12ismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferanidenticalDMEM/F-12productmadeinourScotlandfacility(31330-038).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
ForInVitroDiagnosticUse.