Information Based on immunoelectrophoresis, the antibody reacts with the Fc portion of rat IgG heavy chain but not with the Fab portion of rat immunoglobulins.No antibody was detected against rat IgM or against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.
Usage Physical State: Freeze-dried solid Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity:The antibody was delipidated and ammonium sulfate fractionated from whole antiserum. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Preservative:0.05% Sodium Azide Images & References
This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
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