Description
MEM(MinimumEssentialMedium)isoneofthemostcommonlyusedofallcellculturemedia.MEMcanbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingHeLa,BHK-21,293,HEP-2,HT-1080,MCF-7,fibroblasts,andprimaryratastrocytes.WeofferavarietyofMEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisMEMismodifiedasfollows:With | Without |
•L-glutamine | •HEPES |
Thecompleteformulationisavailable.
UsingMEMMEMwasdevelopedbyHarryEagle,basedonhisearlierformulationofBasalMediumEagle(BME).ManyothermodificationsofMEMfollowed,includingGlasgow’sMEM,MEMα,DMEM,andTemin’sModification.MEMisavailablewithEarle’ssaltsforuseinaCO
2incubator,orwithHanks"saltsforusewithoutCO
2.ThisproductismadewithEarle’ssalts.MEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).MEMusesasodiumbicarbonatebuffersystem(2.2g/L),andthereforerequiresa5–10%CO
2environmenttomaintainphysiologicalpH.
cGMPmanufacturingandqualitysystemMEMismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferanidenticalMEMproductmadeinourScotlandfacility(31095-029).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.