Gibco/DMEM, high glucose, GlutaMAX Supplement, pyruvate/10 L/10569077

Description

DMEM(Dulbecco"sModifiedEagleMedium)isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinDMEMincludeprimaryfibroblasts,neurons,glialcells,HUVECs,andsmoothmusclecells,aswellascelllinessuchasHeLa,293,Cos-7,andPC-12.WeofferavarietyofDMEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
Thecompleteformulationisavailable.

UsingDMEM
DMEMisuniquefromothermediaasitcontains4timestheconcentrationofaminoacidsandvitaminsthantheoriginalEagle"sMinimalEssentialMedium.DMEMwasoriginallyformulatedwithlowglucose(1g/L)andsodiumpyruvate,butisoftenusedwithhigherglucoselevels,withorwithoutsodiumpyruvate.DMEMwithGlutaMAX™supplementminimizestoxicammoniabuild-upandimprovescellviABIlityandgrowthinaneasy-to-useformat.DMEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,DMEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).DMEMusesasodiumbicarbonatebuffersystem(3.7g/L),andthereforerequiresa5–10%CO₂environmenttomaintainphysiologicalpH.

Productuse
Forhumanexvivotissueandcellcultureprocessingapplications.CAUTION:Whenusedasamedicaldevice,Federallawrestrictsthisdevicetosalebyorontheorderofaphysician.CustomersusingGibco®DMEMinamanufacturingprocess,whohaveasubmissionwiththeFDA,mayrequestaletterofauthorizationfromustoreferenceourTypeIIDrugMasterFile(DMF).

cGMPmanufacturingandqualitysystem
DMEMismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferanidenticalDMEMproductmadeinourScotlandfacility(31966-021).ThisfacilityisalsoregisteredwiththeFDAasamedicaldevicemanufacturerandcertifiedtotheISO13485standard.