Application | Anti-acetyl-HistoneH4(Lys16)Antibodyisarabbitpolyclonalantibodyfordetectionofacetyl-HistoneH4(Lys16)alsoknownasH4K16Ac,HistoneH4(acetylK16&hasbeenpublishedandvalidatedinChIP,WB,Mplex,PIA,DB,ChIP-seq. |
KeyApplications | - WesternBlotting
- Multiplexing
- PeptideInhibitionAssay
- DotBlot
- ChIP-seq
- ChromatinImmunoprecipitation(ChIP)
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ApplicationNotes | ImmunocytochemistryAnalysis:A1:500dilutionfromarepresentativelotdetectedAcetyl-HistoneH4(Lys16)inHeLa,A431andNIH/3T3cells. ChromatinImmunoprecipitation(ChIP)Analysis:1µgfromarepresentativelotimmunoprecipitatedAcetyl-HistoneH4(Lys16)in1µLofHeLacelllysate. DotBlotAnalysis:A1:5,000dilutionfromarepresentativelotdetectedAcetyl-HistoneH4(Lys16)inanAbsurance™HistoneH3AntibodySpecificityArray(Cat.No.16-667)andanAbsurance™HistoneH2A,H2B,H4AntibodySpecificityArray(Cat.No.16-665). Multiplexing(MPLX):ThisantibodyspecificallyrecognizeshistoneH4acetylatedonLys16byLuminex®assay. PeptideInhibitionAssay(PIA):ThisantibodypeptideblockedonHeLacellextracts. ChIP-SeqAnalysis:Arepresentativelotofthisantibodywasusedbytwoindependent laboratoriesforChIP-Seq(StromeandAhringerLaboratory,UCSantaCruz/Universityof CambridgeandMitziKurodaLaboratory,HarvardMedicalSchool)SeeEgelhofer,T.A.,etal.(2011). |