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Millipore/07-1238 | Anti-phospho-MARCKS (Ser152/156) Antibody/07-1238/100 µL

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货号: 07-1238

品牌: Millipore

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Description
CatalogueNumber	07-1238
Replaces	AB5879
Description	Anti-phospho-MARCKS(Ser152/156)Antibody
AlternateNames	Myristoylatedalanine-richC-kinasesubstrate ProteinkinaseCsubstrate,80kDaprotein,lightchain
BackgroundInformation	MyristoylatedAlanine-RichC-KinaseSubstrate(MARCKS)isawidelyexpressedsubstrateofPKC.MARCKSisessentialinneuronaldevelopment,asmicedeficientinitdieshortlyafterbirth.Throughitsbasiceffectorregion,MARCKSassociateswithacidicmembranephospholipids,actinfilaments,andcalmodulin.Poly(ADP-ribose)alsobindsthebasiceffectorregion,blockingcalmodulinbinding,membraneassociation,andphosphorylationbyPKC,suggestingthatMARCKSmaybeaneffectoroftheDNA-damagesignaltransducedbypoly(ADP-ribose).

ProductInformation
Format	AffinityPurified
Control	A431CellLysate
Presentation	PurifiedrabbitpolyclonalIgGin10mMHepes,pH7.5,with150mMNaCl,100µg/mLBSAand50%glycerol.

StorageandShippingInformation
StorageConditions	Stableat-20°Cinundilutedforupto6monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.Donotstoreinaself-defrostingfreezer. HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

StorageandShippingInformation

StorageConditions

Stableat-20°Cinundilutedforupto6monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.Donotstoreinaself-defrostingfreezer.
HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

Applications
Application	ThisAnti-phospho-MARCKS(Ser152/156)AntibodyisvalidatedforuseinWB,IH(P)forthedetectionofphospho-MARCKS(Ser152/156).
KeyApplications	WesternBlotting Immunohistochemistry(Paraffin)
ApplicationNotes	Immunohistochemistry(paraffin): Representativeimagesfromapreviouslot. OptimalStainingofMARCKSWithCitratepH6.0EpitopeRetrieval:RatPlacenta Optimalworkingdilutionsmustbedeterminedbytheenduser.

BIOLOGicalInformation
Immunogen	SyntheticpeptidecorrespondingtoaminoacidssurroundingthephosphoSer152/156ofratMARCKS.
Epitope	phosphoSer152/156
Host	Rabbit
Specificity	MARCKS,phosphoSer152/156.
Isotype	IgG
SpeciesReactivity	Rat Human Mouse
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_002356.5
EntrezGeneSummary	TheproteinencodedbythisgeneisasubstrateforproteinkinaseC.Itislocalizedtotheplasmamembraneandisanactinfilamentcrosslinkingprotein.PhosphorylationbyproteinkinaseCorbindingtocalcium-calmodulininhibitsitsassociationwithactinandwiththeplasmamembrane,leADIngtoitspresenceinthecytoplasm.Theproteinisthoughttobeinvolvedincellmotility,phagocytosis,membranetraffickingandmitogenesis.
GeneSymbol	MARCKS MACS 80K-L PRKCSL FLJ14368 phosphomyristin MRACKS PKCSL FLJ90045
Modifications	Phosphorylation
PurificationMethod	AffinityPurfied
UniProtNumber	P29966
UniProtSummary	FUNCTION:SwissProt:P29966#MARCKSisthemostprominentcellularsubstrateforproteinkinaseC.Thisproteinbindscalmodulin,actin,andsynapsin.MARCKSisafilamentous(F)actincross-linkingprotein. SIZE:332aminoacids;31555Da PTM:PhosphorylationbyPKCdisplacesMARCKSfromthemembrane.ItalsoinhibitstheF-actincross-linkingactivity. SIMILARITY:SwissProt:P29966##BelongstotheMARCKSfamily.
MolecularWeight	~80kDa

PhysicochemicalInformation

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MaterialsInformation

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Millipore/07-1238 | Anti-phospho-MARCKS (Ser152/156) Antibody/07-1238/100 µL

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