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Millipore/07-1231 | Anti-Notch1 Antibody, Intracellular/07-1231/200 µL
  • Millipore/07-1231 | Anti-Notch1 Antibody, Intracellular/07-1231/200 µL

Millipore/07-1231 | Anti-Notch1 Antibody, Intracellular/07-1231/200 µL

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货号: 07-1231
品牌: Millipore
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    • Description
      CatalogueNumber07-1231
      Replaces04-1046
      DescriptionAnti-Notch1Antibody,Intracellular
      AlternateNames
      • Neurogeniclocusnotchhomologprotein1precursor
      • Notch(Drosophila)homolog1(translocation-associated)
      • Notch1
      • Notchhomolog1,translocation-associated(Drosophila)
      • Translocation-associatednotchproteinTAN-1
      • Neurogeniclocusnotchhomologprotein1
      BackgroundInformationNotchisafamilyofsingle-passtransmembranereceptorproteinsthataresynthesizedintheendoplasmicreticulumasaninactiveformandareproteolyticallycleavedonanextracellularsitebyafurin-likeconvertase(S1cleavage)inthetrans-golginetworkaftertherecognitionoftheRQRRsequencebeforeitreachestheplasmamembraneasheterodimerstoyieldanactive,ligand-accessIBLeform.TheNotchfamilyiscomprisedof4members(1-4)whoseligandsincludetheDeltaandJaggedfamilyofligands.TheseligandscauseproteolysisofNotchtoliberatetheintracellulardomain.CleavageresultsinaC-terminalfragmentN(TN)andaN-terminalfragmentN(EC).Followingligandbinding,itiscleaved(S2cleavage)byTNF-alphaconvertingenzyme(TACE)toyieldamembrane-associatedintermediatefragmentcalledNotchextracellulartruncation(NEXT).Thisfragmentisthencleavedbypresenilin-dependentgamma-secretasebetweengly1743andval1744(S3cleavage)toreleasetheintracellulardomain(NICD)fromthemembrane.ThatdomaintranslocatestothenucleusandinitiatestranscriptioneventsbybindingtheDNAbindingproteinCSL.Thenotchfamilymembersareinvolvedcelldifferentiationanddevelopment.
      ProductInformation
      FormatSerum
      Control
      • HeLaCellLysate
      PresentationRabbitpolyclonalIgGseruminbuffercontainingin0.02MPotassiumphosphate,0.15MNaClwith0.01%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor6monthsat-20°Cinundilutedaliquotsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.
      HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationUseAnti-Notch1Antibody,Intracellular(RabbitPolyclonalAntibody)validatedinELISA,WB,IHC(P)todetectNotch1alsoknownasNeurogeniclocusnotchhomologprotein1precursor.
      KeyApplications
      • ELISA
      • WesternBlotting
      • Immunohistochemistry(Paraffin)
      ApplicationNotesELISA:1:20,000-1:100,000dilutionfromapreviouslotwasusedinastandardsandwichELISAassayagainstthepeptideimmunogen.
      Optimalworkingdilutionsmustbedeterminedbytheenduser.

      Immunohistochemistry(paraffin):
      Representativetestingfromapreviouslot.
      OptimalStainingIGF-1WithEpitopeRetrieval:Lipoma.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidefromhumanNotch1.
      EpitopeIntracellular
      HostRabbit
      SpecificityNotch1,intracellular.NoreactivityagainsthumanNotch2.
      IsotypeIgG
      SpeciesReactivity
      • Human
      SpeciesReactivityNoteHuman.NoreactivityobservedtomouseNotch.Reactivitywithotherspecieshasnotbeendetermined.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesamemberoftheNotchfamily.MembersofthisType1transmembraneproteinfamilysharestructuralcharacteristicsincludinganextracellulardomainconsistingofmultipleepidermalgrowthfactor-like(EGF)repeats,andanintracellulardomainconsistingofmultiple,differentdomaintypes.Notchfamilymembersplayaroleinavarietyofdevelopmentalprocessesbycontrollingcellfatedecisions.TheNotchsignalingnetworkisanevolutionarilyconservedintercellularsignalingpathwaywhichregulatesinteractionsbetweenphysicallyadjacentcells.InDrosophilia,notchinteractionwithitscell-boundligands(delta,serrate)establishesanintercellularsignalingpathwaythatplaysakeyroleindevelopment.Homologuesofthenotch-ligandshavealsobeenidentifiedinhuman,butpreciseinteractionsbetweentheseligandsandthehumannotchhomologuesremaintobedetermined.Thisproteiniscleavedinthetrans-Golginetwork,andpresentedonthecellsurfaceasaheterodimer.Thisproteinfunctionsasareceptorformembraneboundligands,andmayplaymultiplerolesduringdevelopment.
      GeneSymbol
      • NOTCH1
      • notch1
      • hN1
      • TAN1
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P46531#Functionsasareceptorformembrane-boundligandsJagged1,Jagged2andDelta1toregulatecell-fatedetermination.Uponligandactivationthroughthereleasednotchintracellulardomain(NICD)itformsatranscriptionalactivatorcomplexwithRBP-Jkappaandactivatesgenesoftheenhancerofsplitlocus.Affectstheimplementationofdifferentiation,proliferationandapoptoticprograms.Maybeimportantfornormallymphocytefunction.Inalteredform,maycontributetotransformationorprogressioninsomeT-cellneoplasms.InvolvedinthematurationofbothCD4+andCD8+cellsinthethymus.Maybeimportantforfolliculardifferentiationandpossiblycellfateselectionwithinthefollicle.Duringcerebellardevelopment,mayfunctionasareceptorforneuronalDNERandmaybeinvolvedinthedifferentiationofBergmannglia(Bysimilarity).
      SIZE:2556aminoacids;272500Da
      SUBUNIT:HeterodimerofaC-terminalfragmentN(TM)andanN-terminalfragmentN(EC)whichareprobablylinkedbydisulfidebonds.InteractswithDNER,DTX1,DTX2andRBPSUH.AlsointeractswithMAML1,MAML2andMAML3whichactastranscriptionalcoactivatorsforNOTCH1.
      SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein(Bysimilarity).&Notch1intracellulardomain:Nucleus(Bysimilarity).Note=FollowingproteolyticalprocessingNICDistranslocatedtothenucleus(Bysimilarity).
      TISSUESPECIFICITY:Infetaltissuesmostabundantinspleen,brainstemandlung.Alsopresentinmostadulttissueswhereitisfoundmainlyinlymphoidtissues.
      PTM:Synthesizedintheendoplasmicreticulumasaninactiveformwhichisproteolyticallycleavedbyafurin-likeconvertaseinthetrans-Golginetworkbeforeitreachestheplasmamembranetoyieldanactive,ligand-accessibleform.CleavageresultsinaC-terminalfragmentN(TM)andaN-terminalfragmentN(EC).Followingligandbinding,itiscleavedbyTNF-alphaconvertingenzyme(TACE)toyieldamembrane-associatedintermediatefragmentcallednotchextracellulartruncation(NEXT).Thisfragmentisthencleavedbypresenilindependentgamma-secretasetoreleaseanotch-derivedpeptidecontainingtheintracellulardomain(NICD)fromthemembrane(Bysimilarity).&Phosphorylated(Bysimilarity).
      DISEASE:SwissProt:P46531#NOTCH1truncationisassociatedwithT-cellacutelymphoblasticleukemia.&DefectsinNOTCH1areacauseofaorticvalvedisease[MIM:109730].Thedisorderconsistsofanearlydevelopmentaldefectintheaorticvalveandalaterde-repressionofcalciumdepositionthatcausesprogressiveaorticvalvedisease.CalcificationoftheaorticvalveisthethirdleADIngcauseofheartdiseaseinadults.Theincidenceincreaseswithage,anditisoftenassociatedwithabicUSPidaorticvalvepresentin1-2%ofthepopulation.
      SIMILARITY:SwissProt:P46531##BelongstotheNOTCHfamily.&Contains5ANKrepeats.&Contains36EGF-likedomains.&Contains3LNR(Lin/Notch)repeats.
      MolecularWeight~80kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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