Immunogen | KLH-conjugated,syntheticpeptidecorrespondingtoaminoacids129-137ofhumanHDAC4(C-EHQRKLERH) |
Host | Rabbit |
Specificity | HDAC4 |
Isotype | IgG |
SpeciesReactivity | |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Histonesplayacriticalroleintranscriptionalregulation,cellcycleprogression,anddevelopmentalevents.Histoneacetylation/deacetylationalterschromosomestructureandaffectstranscriptionfactoraccesstoDNA.TheproteinencodedbythisgenebelongstoclassIIofthehistonedeacetylase/acuc/aphafamily.Itpossesseshistonedeacetylaseactivityandrepressestranscriptionwhentetheredtoapromoter.ThisproteindoesnotbindDNAdirectly,butthroughtranscriptionfactorsMEF2CandMEF2D.ItseemstointeractinamultiproteincomplexwithRbAp48andHDAC3. |
GeneSymbol | - HDAC4
- HD4
- HDAC-A
- KIAA0288
- HDACA
- HA6116
|
PurificationMethod | ProteinAchromatography |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P56524#ResponsIBLeforthedeacetylationoflysineresiduesontheN-terminalpartofthecorehistones(H2A,H2B,H3andH4).Histonedeacetylationgivesatagforepigeneticrepressionandplaysanimportantroleintranscriptionalregulation,cellcycleprogressionanddevelopmentalevents.Histonedeacetylasesactviatheformationoflargemultiproteincomplexes.InvolvedinmusclematurationviaitsinteractionwiththemyocyteenhancerfactorssuchasMEF2A,MEF2CandMEF2D. SIZE:1084aminoacids;119070Da SUBUNIT:InteractswithHDAC7(Bysimilarity).Homodimer.HomodimerizationviaitsN-terminaldomain.InteractswithMEF2CandNR2C1.Interactswitha14-3-3chaperoneproteininaphosphorylationdependentmanner.InteractswithBTBD14B(Bysimilarity).InteractswithJARID1B. SUBCELLULARLOCATION:Nucleus.Cytoplasm.Note=Shuttlesbetweenthenucleusandthecytoplasm.Uponmusclecellsdifferentiation,itaccumulatesinthenucleiofmyotubes,suggestingapositiveroleofnuclearHDAC4inmuscledifferentiation.Theexporttocytoplasmdependsontheinteractionwitha14-3-3chaperoneproteinandisduetoitsphosphorylationatSer-246,Ser-467andSer-632byCaMK4.Thenuclearlocalizationprobablydependsonsumoylation. TISSUESPECIFICITY:Ubiquitous. DOMAIN:SwissProt:P56524Thenuclearexportsequencemediatestheshuttlingbetweenthenucleusandthecytoplasm. PTM:PhosphorylatedbyCaMK4atSer-246,Ser-467andSer-632.Phosphorylationatotherresiduesisrequiredfortheinteractionwith14-3-3.&SumoylationonLys-559ispromotedbytheE3SUMO-proteinligaseRANBP2,andpreventedbyphosphorylationbyCaMK4. SIMILARITY:Belongstothehistonedeacetylasefamily.Type2subfamily. |
MolecularWeight | 119kDa |