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Millipore/05-530 | Anti-Rad51 Antibody, clone 3C10/05-530/200 µg
  • Millipore/05-530 | Anti-Rad51 Antibody, clone 3C10/05-530/200 µg

Millipore/05-530 | Anti-Rad51 Antibody, clone 3C10/05-530/200 µg

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货号: 05-530
品牌: Millipore
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    • Description
      CatalogueNumber05-530
      ReplacesMABE515
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-Rad51Antibody,clone3C10
      BackgroundInformationRad51issimilartoRecA,bothbiochemicallyandstructurally.Itpromoteshomologouspairingandstrandexchangewithinaregularnucleoproteinfilament.Inyeast,theRad51familyofrelatedproteinsalsoincludesRad55,Rad57,andDmc1.Inmammaliancells,sevengenesinthisfamilyhavebeenidentified(HsRAD51,XRCC2,XRCC3,RAD51B/hREC2andHsDMC1,RAD51C,andRAD51D).RecentstudiessuggestedthatRad51isinvolvednotonlyinhomologousrecombinationbutalsoincellproliferationregulation.
      ProductInformation
      FormatPurified
      Control
      • HeLacellnuclearextract
      Presentation0.1MTris-glycine,pH7.4,0.15MNaCl,0.05%sodiumazidebeforetheadditionofglycerolto30%
      StorageandShippingInformation
      StorageConditions2yearsat-20°C
      Applications
      ApplicationDetectRad51withAnti-Rad51Antibody,clone3C10(MouseMonoclonalAntibody),thathasbeenshowntoworkinIP&WB.
      KeyApplications
      • Immunoprecipitation
      • WesternBlotting
      BIOLOGicalInformation
      ImmunogenFulllengthhumanRad51
      Cloneclone3C10
      HostMouse
      SpecificityRad51;slightcross-reactivitywithDMC1
      IsotypeIgG1
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgeneisamemberoftheRAD51proteinfamily.RAD51familymembersarehighlysimilartobacterialRecAandSaccharomycescerevisiaeRad51,andareknowntobeinvolvedinthehomologousrecombinationandrepairofDNA.ThisproteincaninteractwiththessDNA-bindingproteinRPAandRAD52,anditisthoughttoplayrolesinhomologouspairingandstrandtransferofDNA.ThisproteinisalsofoundtointeractwithBRCA1andBRCA2,whichmaybeimportantforthecellularresponsetoDNAdamage.BRCA2isshowntoregulateboththeintracellularlocalizationandDNA-bindingABIlityofthisprotein.LossofthesecontrolsfollowingBRCA2inactivationmaybeakeyeventleADIngtogenomicinstabilityandtumOrigenesis.Twoalternativelysplicedtranscriptvariantsofthisgene,whichencodedistinctproteins,havebeenreported.TranscriptvariantsutilizingalternativepolyAsignalsexist.
      GeneSymbol
      • RAD51
      • RAD51L3
      • hRAD51
      • HRAD51
      • RECA
      • RAD51D
      • RAD51A
      • HsT16930
      • BRCC5
      • HsRad51
      • HsRAD51
      PurificationMethodProteinGChromatography
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q06609#MayparticipateinacommonDNAdamageresponsepathwayassociatedwiththeactivationofhomologousrecombinationanddouble-strandbreakrepair.BindstosingleanddoublestrandedDNAandexhibitsDNA-dependentATPaseactivity.UnderwindsduplexDNAandformshelicalnucleoproteinfilaments.
      SIZE:339aminoacids;36966Da
      SUBUNIT:InteractswithBRCA1,BRCA2andeitherdirectlyorindirectlywithp53.InteractswithXRCC3,RAD54LandRAD54B.PartofacomplexwithRAD51CandRAD51B.InteractswithRAD51AP1.InteractswithCHEK1/CHK1,andthismayrequirepriorphosphorylationofCHEK1.
      SUBCELLULARLOCATION:Nucleus.Note=ColocalizeswithRAD51AP1tomultiplenuclearfociuponinductionofDNAdamage.
      TISSUESPECIFICITY:Highlyexpressedintestisandthymus,followedbysmallintestine,placenta,colon,pancreasandovary.Weaklyexpressedinbreast.
      PTM:Phosphorylated.PhosphorylationofThr-309byCHEK1/CHK1mayenhanceassociationwithchromatinatsitesofDNAdamageandpromoteDNArepairbyhomologousrecombination.DISEASE:SwissProt:Q06609#DefectsinRAD51areassociatedwithbreastcancer(BC)[MIM:114480].
      SIMILARITY:SwissProt:Q06609##BelongstotherecAfamily.RAD51subfamily.&Contains1HhHdomain.
      MolecularWeight37kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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