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Millipore/04-1032 | Anti-Neurofilament-66 Antibody, clone EP676Y, rabbit monoclonal/04-1032/100 µL
  • Millipore/04-1032 | Anti-Neurofilament-66 Antibody, clone EP676Y, rabbit monoclonal/04-1032/100 µL

Millipore/04-1032 | Anti-Neurofilament-66 Antibody, clone EP676Y, rabbit monoclonal/04-1032/100 µL

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货号: 04-1032
品牌: Millipore
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    • Description
      CatalogueNumber04-1032
      DescriptionAnti-Neurofilament-66Antibody,cloneEP676Y,rabbitmonoclonal
      AlternateNames
      • 66kDaneurofilamentprotein
      • internexinneuronalintermediatefilamentprotein
      • alpha
      • neurofilament5(66kD)
      • neurofilament-66
      • tax-bindingprotein
      BackgroundInformationNeurofilament-66,alsoknownasα-internexin,isamemberoftheIFfamily.ItisfoundindevelopingneuroblastsandintheCNSofadults.Neurofilament-66isexpressedintheadultbrainatlowerlevelsthanotherneurofilamentproteins.
      ProductInformation
      FormatUnpurified
      Control
      • SHSY5Ycelllysate
      PresentationRabbitMonoclonalinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20ºCfromdateofreceipt.
      HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry(Paraffin)
      • Immunocytochemistry
      • Immunoprecipitation
      • FlowCytometry
      ApplicationNotesImmunohistochemistryAnalysis:A1:250-500dilutionfromapreviouslotdetectedNeurofilament-66inhumanbraintissuebutnotinhumancolon,kidneyoruterus.
      ImmunocytochemistryAnalysis:A1:250-500dilutionfromapreviouslotdetectedNeurofilament-66inPC12cells.
      FlowCytometry:A1:25dilutionofapreviouslotwasusedinflowcytometry.
      ImmunoprecipitationAnalysis:A1:50dilutionfromapreviouslotwasusedinIP.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidecorrespondingtoresiduesinthetaildomainofhumanNeurofilament-66.
      EpitopeTailDomain
      CloneEP676Y
      HostRabbit
      SpecificityThisantibodyrecognizesNeurofilament-66atandaroundthetaildomain.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryNeurofilamentsaretypeIVintermediatefilamentheteropolymerscomposedoflight,medium,andheavychains.Neurofilamentscomprisetheaxoskeletonandtheyfunctionallymaintaintheneuronalcaliber.Theymayalsoplayaroleinintracellulartransporttoaxonsanddendrites.Thisgeneisamemberoftheintermediatefilamentfamilyandisinvolvedinthemorphogenesisofneurons.
      GeneSymbol
      • Alpha-Inx
      • FLJ18662
      • FLJ57501
      • MGC12702
      • NEF5
      • NF-66
      • Neurofilament-66
      • OTTHUMP00000020403
      • TXBP-1
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:Class-IVneuronalintermediatefilamentthatisabletoself-assemble.Itisinvolvedinthemorphogenesisofneurons.ItmayformanindependentstructuralnetworkwithouttheinvolvementofotherneurofilamentsoritmaycooperatewithNF-LtoformthefilamentousbackbonetowhichNF-MandNF-Hattachtoformthecross-bridges.
      TISSUESPECIFICITY:FoundpredominantlyinadultCNS.
      DEVELOPMENTALSTAGE:Expressedinbrainasearlyasthe16thweekofgestation,andincreasedrapidlyandreachedasteadystatelevelbythe18thweekofgestation.
      PTM:O-glycosylatedBysimilarity.
      PhosphorylateduponDNAdamage,probablybyATMorATR.
      SEQUENCESIMILARITIES:Belongstotheintermediatefilamentfamily.
      MolecularWeight~66kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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